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12. Antioxidative activity and
Polyphenol content of some Indian Medicinal Plantsϯ
Penmetcha Sreelakshmi, Somaroutu Laxman Rao, Bogadi S. Krishnamohan,
Sukala Kannababu, Dodda Sundararaju and Gottumukkala V. Subbaraju*
Laila Research Centre, Unit I, Phase
III, Jawahar Autonagar, Vijayawada –520 007, India
subbarajugottumukkala@hotmail.com
(Received 17 November 2005; accepted
02 February 2006)
Abstract:
Polyphenols possess antioxidative properties and occur ubiquitously in
plants. The present study evaluates the total phenolic content and the
antioxidative capacity of Indian Medicinal herbs Acacia catechu,
Acacia nilotica, Aegle marmelos, Albizia lebbeck, Alpinia galanga,
Alpinia officinarum, Cassia tora, Cedrus deodara, Crataeva nurvala,
Emblica officinalis, Ficus religiosa, Mimusops elengi, Plumbago
zeylanica, Ricinus communis, Santalum album, Saraca asoca, Symplocos
racemosa, Tamarindus indica, Tecomella undulata, Terminalia bellerica,
Terminalia arjuna and Terminalia chebula. The antioxidative
activity of extracts of the herbs was determined by superoxide free
radical scavenging with nitroblue tetrazolium (NBT) and 1,1-diphenyl-2-picrylhydrazyl
(DPPH) radical scavenging methods and the total phenolic content was
determined by Folin – Ciocalteu method. The total phenolics of the
extracts ranged from 0.67 to 48.62 g of gallic acid equivalents (GAE)
per 100g of dried plant material. The antioxidative activities (IC50)
of the aqueous methanolic extracts determined by NBT and DPPH methods
are in the range of 1.5 to > 100 µg/mL and 2.8 to >100 µg/mL,
respectively. The study showed good correlation between the total
phenolic content and antioxidative capacity of the plant extracts.
Key words:
Polyphenols; antioxidant activity;
Folin – Ciocalteu; DPPH; NBT.
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