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11. Continuous production
of aroma compounds by immobilized lipase-catalyzed esterification
Mohamed Gargouri1*, Ines Ben
Rejeb1 and Marie Dominique Legoy2
1
Enzyme Engineering Group,
National Institute of Applied Science and Technology (INSAT), Tunis,
Tunisia.
2
Laboratoire de Biotechnologie et de
Chimie Bio-organique, Université de La Rochelle, La Rochelle, France.
mohamed.gargouri@insat.rnu.tn
(Received 25 September
2005; accepted 08 January 2006)
Abstract:
The present paper describes the potential of Rhizomucor javanicus
lipase immobilized onto porous anion exchange resin Duolite A7 and
Candida
antarctica
Novozym lipase B, in the production of flavor esters in continuous
systems. Immobilized lipase from R. javanicus was used in a
packed-bed reactor for the production of isoamyl acetate by
esterification of the corresponding alcohol and acid. The yield of
esterification reaction reached 95 % under 1 mL min-1
substrate flow and 40 °C. The second reaction used the
immobilized Novozym lipase B in continuous intra-esterification of
13-hydroxy-linoleic acid in packed-bed reactor
heated to 30°C with substrate flow fixed at 1 mL min-1
(residence time was then equal to 3 min). The studied continuous
system produced lactone in high yields by reducing the inhibition of
enzyme reaction. The described continuous systems use small quantities
of enzyme and allowed rapid transformation of substrates into esters
obtained in high purity, facilitating an eventual further isolation.
Key Words:
Lipase-catalyzed esterification, packed-bed reactor, immobilization,
lactone, organic media.
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