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Estimation of Pterostilbene in Pterocarpus marsupium Heart wood
and Extracts by HPLC and HPTLCϯ
Katta Vijaykumar1, Papolu B. Murthy1, Sukala
Kannababu1, B. Syamasundar2 And Gottumukkala V.
Subbaraju*1
1 Laila Research Centre, Unit I, Phase
III, Jawahar Autonagar,
Vijayawada – 520 007,
India
e-mail:
subbarajugottumukkala@hotmail.com
2
Department of
Chemistry,
Acharya
Nagarjuna
University, Nagarjuna
Nagar–522 510,India
ϯ Laila Impex Communication # 52
Abstract:
Simple and reproducible methods for estimation of pterostilbene, an
anti-diabetic agent found in Pterocarpus marsupium by high
performance liquid chromatography (HPLC) and high performance thin
layer chromatography (HPTLC), were developed and validated. The
methods showed satisfactory linearity and precision, good recovery and
appropriate limits of detection (LOD) and quantification (LOQ).The
content of pterostilbene was determined and the results obtained by
HPLC and HPTLC methods were in good agreement. The methods developed
are suitable for the quality control applications in Pterocarpus
marsupium heart wood and extracts.
Key words:Pterostilbene, Pterocarpus, marsupium,
HPLC, HPTLC
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