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5.
Simultaneous Determination Water-Soluble Vitamin B9 or
Folic Acid in Mint Vegetable, by HPLC Method Coupled with a Solid
Phase Extraction
Rahmatallah Rahimi,a* Neda Goodarzib
ªDepartment
of chemistry, Iran university of Science and Technology (IUST),
Tehran, Iran
rahimiius96@yahoo.com or
rahimi_rah@iust.ac.ir
bDepartment of chemistry, Islamic Azad University, Saveh
branch, saveh –Iran
Abstract:
We
have applied a quick and convenient method for determining Folic
acid excreted in mint vegetable. Sample-treatment entails SPE
with Sep-Pak C18 (0.9 g) cartridge. The folic acid is a
water-soluble vitamin which was analyzed by HPLC on a Discovery
C-18 150mm×4.6mm
column with 0.1 mol.L-1 KH2PO4
(pH 7.0) – methanol, 90:10, as mobile phase (0.7 mL.min-1)
in isocratic mode. The influence of various analytical
parameters including the pH, sorbent amount, elution factors
(concentration and volume of eluting solution), concentration
and volume of sample solution on the extraction efficiency of
analytes ware investigated.
Key words: Solid phase
extraction (SPE), Mint analysis, High-performance liquid
chromatography (HPLC), Folic acid
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