Abstract: Quantitative analysis of poorly water-soluble drugs involves use of various organic solvents. Sodium benzoate is one of the widely used hydrotropic agents and has demonstrated the enhancement in aqueous solubilities of a large number of poorly water-soluble drugs. In the present investigation 3.5 M sodium benzoate solution has been used as hydrotropic solubilizing agent for poorly water-soluble, Rabeprazole sodium and Itropide Hydrochloride in combined dosage form. An ultraviolet (UV) spectrophotometic method was developed and validated for quantitative determination of   the proposed method is based on simultaneous equation method. The absorption maxima (λmax) of Rabeprazole sodium and Itropide Hydrochloride were 271 nm and 306 nm respectively. The calibration curves were prepared. The proposed method obeys Beers law in concentration range 5-35 µg/ml for   Rabeprazole sodium and Itropide Hydrochloride respectively with correlation coefficients were found to be 0.9999 and 0.9981 respectively. The method was contempt using known concentration of drug and percentage recovery was found in the range of 99 % to 105 %. Analyzing the combination in tablet dosage form, recovery study showed a good agreement in the assay of results. The proposed method is simple, precise, and accurate and can be employed for routine analysis of   Rabeprazole sodium and Itropide Hydrochloride in tablet dosage form. The proposed method was validated statistically by low values of statistical parameters viz. standard deviation, percent coefficient of variation and standard error. The proposed method is new, accurate, simple and economic.


KEYWORDS: Rabeprazole sodium, Itropide HCl, Simultaneous equation, Derivative spectrophotometry method, AUC, Hydrotropic Solubilization.