6.Quantitative determination of amlodipine besylate without
derivatized in pure form and tablet dosage forms with UV
spectrophotometric method
Murat
Ozdemir1, Yucel Kadioglu1,2
1Department
of Analytical Chemistry, Faculty of Pharmacy, Ataturk University,
25240,Erzurum, Turkey
2 Corresponding Author: Tlf: +904422311536,
Fax:+904422360962
E-mail:
yucelkadi@hotmail.com or
yucel@atauni.edu.tr
Abstract:
The present work describes the development and validation of
UV Spectrophotometric method for direct determination of
underivatized amlodipine besylate (ADB) in pure and tablet
dosage forms. The validation parameters of linearity,
precision, accuracy, recovery, specificity, limit of
detection and limit of quantification were studied. The
range of quantification for proposed method was 2-17
mg mL-1. The precision of method was
calculated as the relative standard deviation (RSD) and less
than 2 %, and accuracy (relative error) was better than 6 %
(n = 6). The developed method was successfully applied for
the assay of pharmaceutical dosage forms which do not
require any preliminary separation or treatment of the
samples. The RSD values for Norlopin® tablet (5 mg) and
Norvasc® tablet (5 mg) was found to be less than 2 %. The
results obtained from this method were compared with two
reference method reported in literature and no significant
difference was found statistically (p>0.05).
Keywords : Amlodipine besylate, Tablets, UV
Spectrophotometric method
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