Abstract

The adsorption and interfacial behaviour of difloxacin at a hanging mercury drop electrode (HMDE) were studied using out – of – phase and cyclic voltammetry. The results show that difloxacin was strongly adsorbed at HMDE surface, which is the prerequisite step for applying adsorptive stripping voltammetry. A simple, rapid, reliable and fully validated differential pulse cathodic adsorptive stripping voltammetric procedure has been developed for the determination of the antibacterial difloxacin in bulk form and human urine. The limits of detection (LOD) and quantitation (LOQ) for ultratrace determination of difloxacin were calculated as 7.57 10^-10 and 2.52 10^-9 mol l^-1, at the optimized conditions. The degree of interference from coexisting metal ions and some organic compounds on the adsorptive reduction signal for difloxacin was evaluated.